M. Nabrdalik, A. Rombel-Bryzek, E. Moliszewska
University of Opole, Independent Chair of Biotechnology and Molecular Biology Opole, PL
EVALUATION OF CELLULOLYTIC ACTIVITY OF ASPERGILLUS VERSICOLOR
The biological degradation of cellulose has great importance for the buildings material, such as wooden constructions, carton-gypsum boards and wallpapers. In the study Aspergillus versicolor , isolated from the indoor buildings material, was screened for its ability to synthesize cellulolytic enzymes by optimizing different physico-chemical parameters. In view of maximum growth and cellulase activity on the medium subsequent experiments were carried out on the modification Czapek-Dox medium, to ﬁnd out the inﬂuence of supplementation of different carbon, nitrogen, temperature, pH on growth, secretion of extracellular protein content and cellulase production by A. versicolor at 7th day incubation. The study was recorded for free and immobilised cells. Maximum enzyme production was obtained at 30°C when medium was supplemented with lactose (immobilised cells), glucose (free cells) and NH4 NO3 at pH = 5.0. Processes using free growing cells seem to be more activity than those with immobilised cells. Current study holds potential as the strain reported here could easily be used for the production of cellulase.
Keywords: Aspergillus versicolor , cellulolytic activity, immobilisation